Metabolomics profiling of Tephrosia Purpurea, Cynodon dactylon in dry form and Cynodon dactylon in wet form using Gas Chromatography-Mass Spectrometry and cytotoxicity study by using known antibiotics and plants phytochemicals

Abstract

This study focuses on using Gas Chromatography-Mass Spectrometry (GC-MS) to do a thorough metabolic profiling of Cynodon dactylon. Additionally, it assesses the cytotoxicity of the plant in the presence of many plant phytochemicals and known antibiotics. Its varied biochemical characteristics and medicinal potential make Cynodon dactylon, a commonly used grass species, promising. A complex blend of fatty acids, steroids, terpenoids, and flavonoids was revealed by using GC-MS to identify and quantify several metabolites inside the plant. Bioactive substances that may be responsible for the therapeutic effects of the substance are indicated by the metabolic profile. Once the metabolic study was completed, we evaluated the cytotoxic effects of Cynodon dactylon extracts when combined with various antibiotics and phytochemicals. Using common cell viability tests against different cancer cell lines, cytotoxicity was quantified. major cytotoxic activity was demonstrated by the results.

Keywords: Metabolic, potential, spectroscopy, biochemical, therapeutic, antibiotics, bioactive, cytotoxicity, pharmaceutical